Pillar 2

WP9. Immuno-monitoring, immune profiling

Sample/Species Objective Method/ Technique

PBMC, Splenocytes

Assessment of T cell responses

ELISpot, ICS

Serum

Protein profiling

Olink/Luminex

PBMC, Splenocytes

Transcriptomics

scRNAseq
CITEseq
TCR/BCR

PBMC, Splenocytes

Gene expression analysis

direct RNA hybridization (Nanostring) RNA-Seq bulk

PBMC, Splenocytes Syrian Hamster, Mouse

Assessment of T cell responses

ELISpot, ICS

Serum / Syrian Hamster, Mouse

Assessment of B cell responses

ELISA, MNT (VNT, PRNT 50)